Web Published:

       Researchers at Princeton University have developed a novel technique for the simultaneous identification of transfected cells and cell cycle analysis by flow cytometry. Princeton is currently seeking industrial collaborators to commercialize this technology.

       The green fluorescent protein (GFP) is currently used in assays as a marker to detect the subpopulation of transfected cells. However, there are certain technical limitations to the use of GFP in combination with other protocols such as cell cycle analysis using propidium iodide (PI) and flow cytometry. For example, in cell cycle analysis, the use of ethanol results in a loss of GFP fluorescence as it leaches from the cell. Although prior treatment of the cells with paraformaldehyde helps retain the GFP in the cell following ethanol permeabilization, it precludes optimal staining of the DNA with PI.

       The integral membrane GFP-fusion protein developed at Princeton overcomes these limitations and produces a significant increase in the GFP signal for both the enhanced detection of transfected cells as well as for the simultaneous measurement of cell cycle and transfection. The GFP fusion protein is anchored in the cell membrane and is thus retained in the cell during fixation and/or permeabilization techniques using ethanol and may be used in combination with other techniques to quantify cell cycle analysis (using PI) or other intra- or extra-cellular antigens.

       Patent protection is pending.

For more information please contact:

             William H. Gowen
             Office of Technology Licensing and Intellectual Property
             Princeton University
             4 New South Building
             Princeton, NJ 08544-0036
             (609) 258-6762
             (609) 258-1159 fax

Patent Information:
For Information, Contact:
John Ritter
Princeton University
Andrew Beavis
Robert Kalejta
Amy Brideau
Bruce Banfield