Engineered Cys2His2 zinc fingers for efficient non-G rich Target
Princeton Docket # 12-2803
in the Lewis-Sigler Institute for Integrative Genomics, Princeton University
have developed a novel zinc finger profile library, which provides improvements
to and expands upon the existence of known, artificial zinc fingers.
Use as artificial
transcription factors for a given target within a genome of
Nuclease - Site specific genome editing and
chromatin modifying proteins throughput screening of compound libraries
Research tool to
study gene function
Covers the vast
majority of target sequences at 3 different registers of a
Expand the set of
zinc fingers with known specificity to include CNN, ANN and TNN
number of engineered zinc fingers using alternative
finger nucleases and recombinases have been used to make genomic modification in
human cell lines and many model organisms. Zinc fingers are also used as
artificial transcription factors that have been used to regulate specific genes
within a genome of choice. This novel comprehensive set of zinc fingers could be
used to assemble zinc finger arrays able to target auxiliary domains to almost
any sequence of interest, thereby expanding the total number of targets that one
may go after with this type of engineered protein.
library covers the vast majority of target sequences at 3 different registers of
a protein. This drastically
increases the number of engineered zinc fingers outside of the common Zif268 finger 2 backbone, by engineering
complete sets using finger 1 and finger 3. At all 3 positions/backbones we offer
great improvement over previous data where there is significant enrichment of
most fingers outside of the traditional G-rich context. Moreover, the described
fingers at the N and C-terminal positions allows for the assembly of zinc
fingers where the terminal fingers have been selected in the context under which
they had been evolved. Methods for
zinc finger library assembly along with methods of assembling zinc finger arrays
are also provided.
Noyes, PhD is an Associate Research Scholar, at the Lewis-Sigler Institute for
Integrative Genomics, Department of Molecular Biology at Princeton University.
PhD is a Professor of Computer Science and the Lewis-Sigler
Institute for Integrative Genomic focusing on computational
Persikov, PhD is a Postdoc in the Singh lab who focuses computational approaches
to understanding zinc finger specificity.
status and Commercialization Strategy
protection is pending.
University¿s Office of Technology Licensing is interested in identifying
appropriate partners for the further development and commercialization of this
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docket # 12-2803